RAPDS AS AN AID TO EVALUATE THE GENETIC INTEGRITY OF SOMATIC EMBRYOGENESIS-DERIVED POPULATIONS OF PICEA-MARIANA (MILL) BSP

被引：150

作者：

ISABEL, N

TREMBLAY, L

MICHAUD, M

TREMBLAY, FM

BOUSQUET, J

机构：

[1] Centre de recherche en biologie forestiere, Faculte de foresterie et de geomatique, Université Lavai, Sainte-Foy, G1K 7P4, Québec

来源：

THEORETICAL AND APPLIED GENETICS | 1993年 / 86卷 / 01期

关键词：

RAPD; PICEA-MARIANA; GENETIC MARKERS; GENETIC STABILITY; SOMATIC EMBRYOGENESIS;

D O I：

10.1007/BF00223811

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The usefulness of random amplified polymorphic DNA (RAPD) in assessing the genetic stability of somatic embryogenesis-derived populations of black spruce [Picea mariana (Mill.) B.S.P.] was evaluated. Three arbitrary 11-mer primers were successfully used to.amplify DNA from both in-vivo and in-vitro material. Twenty-five embryogenic cell lines, additional zygotic embryos and megagametophytes from three controlled crosses involving four selected genotypes of black spruce were used for the segregation analysis of RAPD variants. Ten markers were genetically characterized and used to evaluate the genetic stability of somatic embryos derived from three embryogenic cell lines (one cell line per cross, 30 somatic embryos per cell line). No variation was detected within clones. The utilization of RAPD markers both for the assessment of genetic stability of clonal materials and to certify genetic stability throughout the process of somatic embryogenesis is discussed.

引用

页码：81 / 87

页数：7

共 46 条

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