ONCOGENE ECT2 IS RELATED TO REGULATORS OF SMALL GTP-BINDING PROTEINS

被引:264
作者
MIKI, T
SMITH, CL
LONG, JE
EVA, A
FLEMING, TP
机构
[1] Laboratory of Cellular and Molecular Biology, National Cancer Institute, Building 37-1E24, Bethesda, MD 20892
[2] Department of Opthalmology and Visual Sciences, Washington University School of Medicine, St. Louis, MO 63110
关键词
D O I
10.1038/362462a0
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
WE have developed an efficient expression cloning system that allows rapid isolation of complementary DNAs able to induce the transformed phenotype1,2. We searched for molecules expressed in epithelial cells and possessing transforming potential to fibroblasts, and cloned a cDNA for the normal receptor of a growth factor secreted by NIH/3T3 cells3,4. Here we report a second novel transforming gene, ect2. The isolated cDNA is activated by amino-terminal truncation of the normal product. The Ect2 protein has sequence similarity within a central core of 255 amino acids with the products of the breakpoint cluster gene, bcr (ref. 5), the yeast cell cycle gene, CDC24 (ref. 6), and the dbl oncogene7. Each of these genes encodes regulatory molecules or effectors for Rho-like small GTP-binding proteins8-10. The baculovirus-expressed Ect2 protein could bind highly specifically to Rho and Rac proteins, whereas the dbl product showed broader binding specificity to Rho family proteins. Thus ect2 is a new member of an expanding family, whose products have transforming properties and interact with Rho-like proteins of the Ras superfamily.
引用
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页码:462 / 465
页数:4
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