POLY(ETHYLENE) GLYCOL MONOMETHYL ETHERS - AN ALTERNATIVE TO POLY(ETHYLENE) GLYCOLS IN PROTEIN CRYSTALLIZATION

被引:25
作者
BRZOZOWSKI, AM [1 ]
TOLLEY, SP [1 ]
机构
[1] UNIV LODZ,DEPT CRYSTALLOG,PL-90236 LODZ,POLAND
来源
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 1994年 / 50卷
关键词
D O I
10.1107/S090744499400199X
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Poly(ethylene) glycol monomethyl ethers (Peg-mmes) are a series of methyl substituted poly(ethylene) glycols that have been used with some success in the crystallization of a number of hydrophobic proteins. Crystallization of a lipase from Humicola lanuginosa complexed with the C12 substrate analogue from Peg-mme 5000, an endoglucanase 1 and a 59 kDa fragment of human topoisomerase Ila crystallized from Peg-mme are described. The use of Peg-mme for improving the quality of crystals previously grown from normal poly(ethylene) glycol 8000 is also described. We suggest that these modified Peg-mmes should be regularly used in screening for crystallization.
引用
收藏
页码:466 / 468
页数:3
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