CHARACTERIZATION BY H-1-NMR SPECTROSCOPY OF OLIGOSACCHARIDES DERIVED FROM ALKALI-EXTRACTABLE WHEAT-FLOUR ARABINOXYLAN BY DIGESTION WITH ENDO-(1-]4)-BETA-D-XYLANASE-III FROM ASPERGILLUS-AWAMORI

被引：44

作者：

KORMELINK, FJM

HOFFMANN, RA

GRUPPEN, H

VORAGEN, AGJ

KAMERLING, JP

VLIEGENTHART, JFG

机构：

[1] AGR UNIV WAGENINGEN,BIOTECHNION,DEPT FOOD SCI,POB 8129,6700 EV WAGENINGEN,NETHERLANDS

[2] UNIV UTRECHT,BIJVOET CTR,DEPT BIOORGAN CHEM,3508 TB UTRECHT,NETHERLANDS

来源：

CARBOHYDRATE RESEARCH | 1993年 / 249卷 / 02期

关键词：

D O I：

10.1016/0008-6215(93)84101-B

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Alkali-extractable wheat-flour arabinoxylan, treated with endo-(1 --> 4)-beta-D-xylanase III from Aspergillus awamori CMI 142717, was fractionated by Bio-Gel P-2 size exclusion chromatography at 60-degrees-C. Column fractions, corresponding to oligosaccharides with degrees of polymerisation from 5 to 10, were collected, and subfractionated by high performance anion-exchange chromatography on CarboPac PA-1. The structures of the oligosaccharides thus obtained were elucidated by H-1 NMR spectroscopy, showing chains of (1 --> 4)-linked beta-D-xylopyranosyl residues differently substituted at O-3 and/or O-2,3 with alpha-L-arabinofuranosyl groups. The structures were different from those obtained with endo-(1 --> 4)-beta-D-xylanase I of the same xylanolytic enzyme system.

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页码：369 / 382

页数：14

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