CLONING AND NUCLEOTIDE-SEQUENCE OF FOSFOMYCIN BIOSYNTHETIC GENES OF STREPTOMYCES-WEDMORENSIS

被引：66

作者：

HIDAKA, T

GODA, M

KUZUYAMA, T

TAKEI, N

HIDAKA, M

SETO, H

机构：

[1] UNIV TOKYO,INST MOLEC & CELLULAR BIOSCI,BUNKYO KU,TOKYO 113,JAPAN

[2] UNIV TOKYO,DEPT BIOTECHNOL,DIV AGR & AGR LIFE SCI,BUNKYO KU,TOKYO 113,JAPAN

来源：

MOLECULAR & GENERAL GENETICS | 1995年 / 249卷 / 03期

关键词：

FOSFOMYCIN; C-P BOND;

D O I：

10.1007/BF00290527

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The biosynthetic pathway for production of the antibiotic fosfomycin by Streptomyces wedmorensis consists of four steps including the formation of a C-P bond and an epoxide. Fosfomycin production genes were cloned from genomic DNA using S. wedmorensis mutants blocked at different steps of the biosynthetic pathway. Four genes corresponding to each of the biosynthetic steps were found to be clustered in a DNA fragment of about 5 kb. Nucleotide sequencing of a large fragment revealed the presence of ten open reading frames, including the four biosynthetic genes and six genes with unknown functions.

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页码：274 / 280

页数：7

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