FACILE OPTICAL RESOLUTION OF AMINO-ACID ESTERS VIA HYDROLYSIS BY AN INDUSTRIAL ENZYME IN ORGANIC-SOLVENTS

被引：16

作者：

KIJIMA, T ^{[1
]}

OHSHIMA, K ^{[1
]}

KISE, H ^{[1
]}

机构：

[1] UNIV TSUKUBA,INST MAT SCI,TSUKUBA,IBARAKI 305,JAPAN

来源：

JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY | 1994年 / 59卷 / 01期

关键词：

OPTICAL RESOLUTION; AMINO ACID ESTER; HYDROLYSIS; ALCALASE; PROTEASE; ORGANIC SOLVENT;

D O I：

10.1002/jctb.280590110

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Racemic amino acid esters were optically resolved via hydrolysis in organic solvents by the catalysis of an industrial alkaline protease, 'Alcalase'. The products which were composed mainly of L-amino acids were insoluble and easily separated by filtration. The activity of the enzyme and enantiomeric excess of the products were significantly dependent on the nature of solvent and the water content in the reaction media. Generally, high values of enantiomeric excess were obtained at low water contents. Many natural and unnatural amino acids were resolved by this method.

引用

页码：61 / 65

页数：5

共 18 条

[1] RESOLUTION OF (+/-)-TRANS-SOBREROL BY LIPASE PS-CATALYZED TRANSESTERIFICATION AND EFFECTS OF ORGANIC-SOLVENTS ON ENANTIOSELECTIVITY [J].