LIPOFECTION OF SYNTHETIC OLIGODEOXYRIBONUCLEOTIDE HAVING A PALINDROMIC SEQUENCE OF AACGTT TO MURINE SPLENOCYTES ENHANCES INTERFERON-PRODUCTION AND NATURAL-KILLER ACTIVITY

被引：90

作者：

YAMAMOTO, T

YAMAMOTO, S

KATAOKA, T

TOKUNAGA, T

机构：

[1] Department of Bacterial and Blood Products, Musashi-Murayama

[2] National Institute of Health, Musashi-Murayama

来源：

MICROBIOLOGY AND IMMUNOLOGY | 1994年 / 38卷 / 10期

关键词：

SYNTHETIC OLIGODNA; PALINDROME; LIPOSOME; IFN PRODUCTION;

D O I：

10.1111/j.1348-0421.1994.tb01867.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A synthetic 22-mer oligodeoxyribonucleotide having an AACGTT palindrome, AAC-22, induced interferon (IFN) production and augmented the natural killer (NK) activity in murine splenocytes, whereas its analogue, ACC-22, having an ACCGGT palindrome, did not. The binding of AAC-22 to splenocytes was not different from that of ACC-22. Lipofection of AAC-22 to splenocytes remarkably enhanced IFN production and NK cell activity, whereas that of ACC-22 caused little enhancement. These results strongly suggest that the prerequisite for IFN production is not the binding of AAC-22 to the cell surface receptors, but its penetration into the spleen cells.

引用

收藏

页码：831 / 836

页数：6

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