A SIMPLE, SPECIFIC, AND HIGHLY SENSITIVE BLOCKING ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR DETECTION OF ANTIBODIES TO BOVINE HERPESVIRUS-1

被引：111

作者：

KRAMPS, JA ^{[1
]}

MAGDALENA, J ^{[1
]}

QUAK, J ^{[1
]}

WEERDMEESTER, K ^{[1
]}

KAASHOEK, MJ ^{[1
]}

MARISVELDHUIS, MA ^{[1
]}

RIJSEWIJK, FAM ^{[1
]}

KEIL, G ^{[1
]}

VANOIRSCHOT, JT ^{[1
]}

机构：

[1] FED RES CTR VIRUS DIS ANIM, W-7400 TUBINGEN, GERMANY

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1994年 / 32卷 / 09期

关键词：

D O I：

10.1128/JCM.32.9.2175-2181.1994

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

By using a monoclonal antibody directed against an epitope located on glycoprotein B of bovine herpesvirus 1 (BHV1), a simple, convenient blocking enzyme-linked immunosorbent assay (ELISA) which combines a high sensitivity with a low false-positive rate has been developed. The test can be performed at low variance on undiluted bovine serum samples. The epitope on glycoprotein B appears to be conserved, because it could be detected by immunostaining in all of 160 BHV1 isolates originating from 10 countries. In testing 215 anti-BHV1 antibody-negative and 179 anti-BHV1 antibody-positive serum samples, specificity and sensitivity were 0.96 and 0.99, respectively. This blocking ELISA is superior to a commercially available indirect ELISA and to the 24-h virus neutralization test in detecting low antibody levels in serum. In addition, this blocking ELISA is able to detect specific antibodies in serum as early as 7 days postinfection. To minimize any risk of introducing latent BHV1 carriers among noninfected cattle, this blocking ELISA would be, in our opinion, the test of choice.

引用

页码：2175 / 2181

页数：7

共 36 条

[21] CHARACTERIZATION OF ENVELOPE PROTEINS OF INFECTIOUS BOVINE-RHINOTRACHEITIS VIRUS (BOVINE HERPESVIRUS-1) BY BIOCHEMICAL AND IMMUNOLOGICAL METHODS [J].