A UBIQUITOUS PROTEIN IS THE SOURCE OF NATURALLY-OCCURRING PEPTIDES THAT ARE RECOGNIZED BY A CD8+ T-CELL CLONE

被引：134

作者：

UDAKA, K ^{[1
]}

TSOMIDES, TJ ^{[1
]}

WALDEN, P ^{[1
]}

FUKUSEN, N ^{[1
]}

EISEN, HN ^{[1
]}

机构：

[1] MAX PLANCK INST BIOL,W-7400 TUBINGEN,GERMANY

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1993年 / 90卷 / 23期

关键词：

T-CELL RECEPTOR; MAJOR HISTOCOMPATIBILITY COMPLEX; ALLOREACTIVITY; 2-OXOGLUTARATE DEHYDROGENASE; PROTEASOMES;

D O I：

10.1073/pnas.90.23.11272

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We previously isolated from mouse spleen an octapeptide (LSPFPFDL) that in association with the class I major histocompatibility complex protein L(d) is recognized by the antigen-specific receptor of an alloreactive CD8+ T-cell clone (2C). Guided by an assay dependent upon the same 2C T-cell receptor, we have now isolated from the same source another naturally occurring peptide. The second peptide (VAITRIEQLSPFPFDL) includes the entire octapeptide sequence and preliminary evidence suggests that it may be a natural precursor of the octapeptide. On finding extensive sequence homology between the 16-mer and part of human 2-oxoglutarate dehydrogenase, we determined the cDNA sequence of mouse 2-oxoglutarate dehydrogenase and found that the deduced amino acid sequence matches precisely the two naturally occurring peptides, indicating their origin by cellular processing of this ubiquitous self protein.

引用

页码：11272 / 11276

页数：5

共 28 条

[1]

ALEXANDER J, 1990, IMMUNOGENETICS, V31, P169

[2] PROTEASOME SUBUNITS ENCODED IN THE MHC ARE NOT GENERALLY REQUIRED FOR THE PROCESSING OF PEPTIDES BOUND BY MHC CLASS-I MOLECULES [J].