PARTIAL-PURIFICATION OF A LIPOXYGENASE FROM APPLES

被引：42

作者：

KIM, IS ^{[1
]}

GROSCH, W ^{[1
]}

机构：

[1] DEUTSCHE FORSCHUNGSANSTALT LEBENSMITTELCHEM,D-8046 GARCHING,FED REP GER

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1979年 / 27卷 / 02期

关键词：

D O I：

10.1021/jf60222a022

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

A membrane-bound lipoxygenase (EC 1.13.11.12) was partially purified from apples by differential centrifugation and gel chromatography. The enzyme had a pH optimum at 6.0 and converted linoleic acid predominantly into the 13-hydroperoxyoctadeca-9, ll-dienoic acid. Reversible inhibition was obtained with ethylenediaminetetraacetic acid disodium salt and cyanide. Hemoproteins were not involved in the lipid peroxidation activity. © 1979, American Chemical Society. All rights reserved.

引用

页码：243 / 246

页数：4

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