EFFECTOR-MEDIATED STIMULATION OF ATPASE ACTIVITY BY THE SIGMA(54)-DEPENDENT TRANSCRIPTIONAL ACTIVATOR FHLA FROM ESCHERICHIA-COLI

The FHLA protein is the transcriptional regulator of the genes of the formate regulon from Escherichia coli, The protein shares homology with the sigma(54)-dependent regulators of the NTRC family in the central and C-terminal domains but differs in possessing an extended N terminus lacking the aspartate residue which is the site of phosphorylation. Purified FHLA displays intrinsic ATPase activity which is stimulated weakly by formate and DNA, The presence of both formate and DNA carrying the upstream regulatory sequence to which FHLA binds leads to a large increase in the rate of ATP hydrolysis, Hypophosphite, a structural analog of formate, and azide, a transition state analog of formate, also stimulate ATPase activity, supporting the conclusion that formate is a direct ligand of FHLA, Half-maximal saturation of FHLA,vith formate took place at around 5 mM, and half-maximal saturation with target DNA took place at around 50 nM, The stimulation of ATPase activity by formate was conferred by a decrease in the apparent K-m for ATP, whereas the effect of the DNA binding site also affected the K-cat of the reaction, The other nucleoside triphosphates, GTP, UTP, and CTP, competed with ATP cleavage by FHLA, suggesting at least their binding to FHLA. The specific ATPase activity of FHLA was dependent on the concentration of FHLA in the assay, especially in the presence of DNA and formate, Direct liganding of the effector, therefore, leads to the same consequence as phosphorylation for the NTRC-type regulators, namely, stimulation of ATPase activity.