GALACTOSYLATION BY USE OF BETA-GALACTOSIDASE - ENZYMATIC SYNTHESES OF DISACCHARIDE NUCLEOSIDES

被引:23
作者
BINDER, WH
KAHLIG, H
SCHMID, W
机构
[1] Institut für Organische Chemie der Universität Wien, A-1090 Vienna
关键词
D O I
10.1016/0957-4166(95)00216-C
中图分类号
O61 [无机化学];
学科分类号
070301 ; 081704 ;
摘要
The synthesis of various galactose containing disaccharide nucleosides has been achieved by utilizing the transgalactosylation potential of beta-galactosidase from Aspergillus oryzae. Thus, using p-nitrophenyl-beta-D-galactoside 1 as galactosyl donor, 2-deoxyuridine 2a, uridine 2b, thymidine 2c and adenosine 2d have proven to be useful accepters for the enzyme catalyzed disaccharide nucleoside formation. The regiochemistry of the products 4a - 4d formed after acetylation has been assigned unambiguously by using modem NMR-techniques.
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页码:1703 / 1710
页数:8
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