INTERACTION OF MG(II), CA(II) AND MN(II) IONS WITH THE LIGHT-HARVESTING PROTEINS OF CHLOROPLAST THYLAKOID MEMBRANES STUDIED BY FT-IR DIFFERENCE SPECTROSCOPY

被引：11

作者：

AHMED, A ^{[1
]}

TAJMIRRIAHI, HA ^{[1
]}

机构：

[1] UNIV QUEBEC,CTR RECH PHOTOBIOPHYS,CP 500,TROIS RIVIERES G9A 5H7,PQ,CANADA

来源：

JOURNAL OF MOLECULAR STRUCTURE | 1994年 / 319卷

关键词：

D O I：

10.1016/0022-2860(93)07937-R

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The interaction of Mg(II), Ca(II) and Mn(II) ions with the light-harvesting (LHC-II) proteins of chloroplast thylakoid membranes was investigated in aqueous solution at different metal ion concentrations (0.01-20 mM), using Fourier transform-infrared (FT-IR) difference spectroscopy. The infrared difference spectroscopic results for the amide I and amide II regions (1800-1500 cm-1) have shown a strong metal-protein interaction at high metal ion concentrations (5-20 mM), whereas at very low concentrations (0.01-1 mM) the metal cation binding is negligibie. The metal ion binding is mainly via the protein carbonyl group at low cation concentration, whereas metal ion coordination to the protein C=O and C-N groups were observed at higher cation concentrations. The Mn-tyrosine binding was also observed at high metal ion concentrations. Major conformational changes from alpha-helix (48% in uncomplexed protein) to beta-sheet and turn structures were observed in the presence of these metal cations at high concentrations (10-20 mM).

引用

页码：145 / 151

页数：7

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