STABILITY OF THE LIPASE IMMOBILIZED ON DEAE-SEPHADEX FOR CONTINUOUS LIPID HYDROLYSIS IN ORGANIC-SOLVENT

被引：17

作者：

YANG, D ^{[1
]}

RHEE, JS ^{[1
]}

机构：

[1] KOREA INST SCI & TECHNOL, DEPT BIOL SCI & ENGN, POB 150, SEOUL 130650, SOUTH KOREA

来源：

BIOTECHNOLOGY LETTERS | 1991年 / 13卷 / 08期

关键词：

D O I：

10.1007/BF01033408

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Continuous hydrolysis reaction was carried out in glass-column by using lipase from Candida rugosa immobilized on DEAE-Sephadex A50. Substrate olive oil dissolved in hydrophobic organic solvent (isooctane) was supplied into the reactor with a concurrent stream of aqueous buffer. Stability of the immobilized enzyme was greatly increased in higher substrate concentration. Glycerol added in the aqueous stream showed stabilizing effect against the organic solvent; half life was extended from 220 hrs to 450 hrs by 15% glycerol supplemented at 30-degrees-C.

引用

页码：553 / 558

页数：6

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