DETERMINATION OF ISOPRENALINE WITH LUCIGENIN CHEMILUMINESCENCE USING FLOW-INJECTION ANALYSIS

A procedure is presented for determining isoprenaline (10(-7) - 10(-4) M) in a flow injection system using chemiluminescence detection resulting from the reaction of isoprenaline with 10,10'-dimethyl-9,9-biacridinium dinitrate (lucigenin) in aqueous solution of potassium hydroxide as a carrier stream. The logarithmic calibration curve was linear over the range from 1 X 10(-7) M to 1 X 10(-4) M. The effects of some analytical conditions on the chemiluminescence yield were investigated. The RSD at 1 X 10(-4) isoprenaline is 0.9% (10 replicates). The method is directly applicable to aqueous solutions of isoprenaline injection (1 mg/5 ml).