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TRANSFORMATION OF MAIZE (ZEA-MAYS L) PROTOPLASTS AND REGENERATION OF HAPLOID TRANSGENIC PLANTS

被引：12
作者：
SUKHAPINDA, K
KOZUCH, ME
RUBINWILSON, B
AINLEY, WM
MERLO, DJ
机构：
[1] DowElanco Biotechnology Laboratory, Indianapolis, 46268-1053, IN
来源：
PLANT CELL REPORTS | 1993年 / 13卷 / 02期
关键词：
ZEA-MAYS L; MICROSPORE-DERIVED CULTURES; HAPLOID; REGENERATION; TRANSFORMATION;
D O I：
10.1007/BF00235291
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Transgenic haploid maize (Zea mays L.) plants were obtained from protoplasts isolated from microspore-derived cell suspension cultures. Protoplasts were electroporated in the presence of plasmid DNA containing the gus A and npt II genes encoding B-glucuronidase (GUS) and neomycin phosphotransferase II (NPT II), respectively. Transformed calli were selected and continuously maintained on kanamycin containing medium. Stable transformation was confirmed by enzyme assays and DNA. analysis. Stably transformed tissue was transferred to regeneration medium and several plants were obtained. Most plants showed NPF II activity, and some also showed GUS activity. Chromosome examinations performed on representative plants showed that they were haploid. As expected, these plants were infertile.
引用
收藏
页码：63 / 68
页数：6
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