3-DIMENSIONAL RECONSTRUCTION OF SINGLE PARTICLES NEGATIVELY STAINED OR IN VITREOUS ICE

被引：48

作者：

FRANK, J ^{[1
]}

RADERMACHER, M ^{[1
]}

机构：

[1] SUNY ALBANY, DEPT BIOMED SCI, ALBANY, NY 12222 USA

来源：

ULTRAMICROSCOPY | 1992年 / 46卷 / 1-4期

关键词：

D O I：

10.1016/0304-3991(92)90018-F

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

The random-conical reconstruction method has been highly successful in three-dimensional imaging of macromolecules under low-dose conditions. This article summarizes the different steps of this technique as applied to molecules prepared with negative staining or vitreous ice, and sketches out the current directions of development. We anticipate that by using new instrumental developments, transfer function correction and computational refinement techniques, a resolution in the range of 7-10 angstrom could ultimately be achieved.

引用

页码：241 / 262

页数：22

共 76 条

[41] A NEW 3-D RECONSTRUCTION SCHEME APPLIED TO THE 50S RIBOSOMAL-SUBUNIT OF ESCHERICHIA-COLI [J].