EFFECT OF TEMPERATURE ON SEPARATION OF NORGESTREL ENANTIOMERS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：31

作者：

LAMPARCZYK, H

ZARZYCKI, PK

NOWAKOWSKA, J

机构：

[1] Medical Academy, Faculty of Pharmacy, PL-80-416 Gdańisk

来源：

JOURNAL OF CHROMATOGRAPHY A | 1994年 / 668卷 / 02期

关键词：

D O I：

10.1016/0021-9673(94)80133-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The influence of mobile phase composition, concentration of beta-cyclodextrin and temperature on the high-performance liquid chromatographic separation of norgestrel was studied. In studies of the effect of temperature on the enantioselectivity of (+/-)-norgestrel, acetonitrile-water (25:75, v/v) modified by the addition of beta-cyclodextrin (14 mM) was applied as the mobile phase. Enantiomers were detected using UV detection at 240 nm. The capacity factors were measured over a wide range of column temperatures from -5 to 70 degrees C.

引用

页码：413 / 417

页数：5

共 15 条

[11]

Snyder L.R., 1970, J CHROMATOGR SCI, V8, P692, DOI [10.1093/chromsci/8.12.692, DOI 10.1093/CHROMSCI/8.12.692]

[12] ALPHA-CYCLODEXTRIN AS SELECTIVE AGENT FOR THE SEPARATION OF ORTHO-NITROBENZOIC, META-NITROBENZOIC AND PARA-NITROBENZOIC ACIDS BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].