WHAT SPECIES IS RESPONSIBLE FOR STRAND SCISSION IN THE REACTION OF [FE(II)EDTA](2-) AND H2O2 WITH DNA

被引:142
作者
POGOZELSKI, WK [1 ]
MCNEESE, TJ [1 ]
TULLIUS, TD [1 ]
机构
[1] JOHNS HOPKINS UNIV,DEPT CHEM,BALTIMORE,MD 21218
关键词
D O I
10.1021/ja00129a002
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
The reaction of ferrous iron with hydrogen peroxide (the Fenton reaction) has been widely used as a means of inducing strand cleavage in DNA, generally for the purpose of ''footprinting'' protein-DNA complexes or studying DNA structure. The identity of the DNA-oxidizing species produced in the reaction, however, has been an issue of debate. By comparing gamma-radiolysis-induced DNA cleavage patterns in a variety of buffers with those generated by the reaction of [Fe(EDTA)](2-) + H2O2 in the presence of ascorbate, we report that under the conditions used for a typical footprinting experiment, results are consistent with production of the hydroxyl radical as the oxidant responsible for DNA strand scission. We also cite experiments that argue against the participation of a high-valent iron-ore complex in DNA cutting.
引用
收藏
页码:6428 / 6433
页数:6
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