STRATEGIES FOR OPTIMIZING SERUM-FREE MEDIA

被引：23

作者：

HEWLETT, G

机构：

[1] Institute for Virology, Bayer AG, Wuppertal

来源：

CYTOTECHNOLOGY | 1991年 / 5卷 / 01期

关键词：

LIPOPROTEIN; SELENIUM; ALBUMIN; TRANSFERRIN; INSULIN; FIBRONECTIN;

D O I：

10.1007/BF00365530

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Two of the main reasons for developing serum-free medium are to improve the growth and to increase the productivity of a cell. This review has attetmpted to show that these goals can be achieved far more easily than generally realised by utilising the methods that have been developed during the past decade and by using a limited number of standard supplements. Serum-free media have contributed greatly to the advances made in recent years in the fields of cell biology and mammalian cell technology and it is clear that they will continue to play a major role in the clarification of cellular regulatory processes and in the refinement of processes in modern cyto-technology. © 1991 Kluwer Academic Publishers.

引用

页码：3 / 14

页数：12

共 38 条

[21]

Katsuta H., Takaoka T., Hosaka S., Hibino M., Otsuki I., Hattori K., Suzuki S., Mitamura K., Protein requirements of rat ascites hepatoma cells in tissue culture, Jpn. J. Exp. Med., 29, pp. 45-70, (1959)

[22]

MacLeod A.J., The use of plasma protein fractions as medium supplements for animal cell culture, Adv. Biochem. Eng., 37, pp. 41-56, (1988)

[23]

Maurer H.R., Towards chemically-defined, serum-free media for mammalian cell culture, Animal Cell Culture a practical approach, pp. 13-31, (1986)

[24]

Metzger J.F., Fusilio M.H., Cornman I., Kuhns D.M., Antibiotics in tissue culture, Exp. Cell. Res., 6, pp. 337-344, (1954)

[25]

Mizrahi A., Pluronic polyols in human lymphocyte cell line cultures, J. Clin. Microbiol., 2, pp. 11-13, (1975)

[26]

Moellering W.J., Tedesco J.L., Townsend R.R., Hardy, Scott R.W., Prior C.P., Electrophoretic differences in a MAb expressed in three media, Biopharm., 3, pp. 30-38, (1990)

[27]

Murakami H., Okazaki Y., Yamada K., Omura H., Egg yolk lipoprotein, a new supplement for the growth of mammalian cells in serum-free medium, Cytotechnology, 1, pp. 159-169, (1988)

[28]

Murakami H., What should be focused in the study of cell culture technology for production of bioactive proteins, Cytotechnology, 3, pp. 3-7, (1990)

[29]

Murhammer D.W., Goochee C.F., Scale-up of insect cell cultures: protective effects of pluronic F68. Beta-galactoside production in Spodoptera frugiperda culture, Bio/ Technology, 6, pp. 1411-1415, (1988)

[30]

Paul J., Cell and Tissue Culture, (1959)

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