STRATEGIES FOR OPTIMIZING SERUM-FREE MEDIA

被引：23

作者：

HEWLETT, G

机构：

[1] Institute for Virology, Bayer AG, Wuppertal

来源：

CYTOTECHNOLOGY | 1991年 / 5卷 / 01期

关键词：

LIPOPROTEIN; SELENIUM; ALBUMIN; TRANSFERRIN; INSULIN; FIBRONECTIN;

D O I：

10.1007/BF00365530

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Two of the main reasons for developing serum-free medium are to improve the growth and to increase the productivity of a cell. This review has attetmpted to show that these goals can be achieved far more easily than generally realised by utilising the methods that have been developed during the past decade and by using a limited number of standard supplements. Serum-free media have contributed greatly to the advances made in recent years in the fields of cell biology and mammalian cell technology and it is clear that they will continue to play a major role in the clarification of cellular regulatory processes and in the refinement of processes in modern cyto-technology. © 1991 Kluwer Academic Publishers.

引用

页码：3 / 14

页数：12

共 38 条

[31]

Riss T.L., Karey K.P., Burleigh B.D., Parker D., Sirbasku D.A., Human recombinant insulin-like growth factor I. I. Development of a serum-free medium for clonal density assay of growth factors using Balb/c 3T3 mouse embryo fibroblasts, In Vitro Cell. Dev. Biol., 24, pp. 1099-1106, (1988)

[32]

Rockwell G.A., Growth of SV40 Balb/c-3T3 cells in serum-free culture medium, Cell Culture Methods for Molecular and Cell Biology, Vol. 3, pp. 221-231, (1984)

[33]

Saier M.H., Hormonally defined, serum-free medium for a proximal tubular kidney epithelial cell line, LLC-PK1, Cell Culture Methods for Molecular and Cell Biology, Vol. 3, pp. 25-31, (1984)

[34]

Shintani Y., Iwamoto K., Kitano K., Polyethylene glycols for promoting the growth of mammalian cells, Appl. Microbiol. Biotechnol., 27, pp. 533-537, (1988)

[35]

Swim H.E., Parker R.F., Effect of pluronic F68 on growth of fibroblasts in suspension on a rotary shaker, Proc. Sci. Exp. Biol. Med., 103, pp. 252-254, (1960)

[36]

Taub M., Growth of primary and established kidney cell cultures in serum-free media, Cell Culture Methods for Molecular and Cell Biology, Vol. 3, pp. 3-24, (1984)

[37]

Walthall B.J., Ham R.G., Multiplication of human diploid fibroblasts in a synthetic medium supplemented with EGF, insulin and dexamethasone, Exp. Cell Res., 134, pp. 303-311, (1981)

[38]

Waymouth C., Preparation and use of serum-free culture media, Cell Culture Methods for Molecular and Cell Biology, Vol. 1, pp. 23-68, (1984)

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