SENSITIVE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF THE 3 MAIN METABOLITES OF SELEGILINE (L-DEPRENYL) IN HUMAN PLASMA

被引：15

作者：

LACROIX, R ^{[1
]}

PIANEZZOLA, E ^{[1
]}

BENEDETTI, S ^{[1
]}

机构：

[1] PHARM FARM CARLO ERBA,RES & DEV,DEPT METAB & PHARMACOKINET,I-20014 NERVIANO,ITALY

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1994年 / 656卷 / 01期

关键词：

D O I：

10.1016/0378-4347(94)00039-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A high-performance liquid chromatographic (HPLC) method with fluoresence detection was developed for the determination in human plasma of the three main metabolites of selegiline (L-deprenyl): amphetamine, methamphetamine and norselegiline. The HPLC separation of the analytes was performed under isocratic conditions, after extraction from plasma and precolumn derivatization with 9-fluorenylmethyl chloroformate. The linearity, precision and accuracy of the method were evaluated; the limit of quantification for all three metabolites in plasma was 0.5 ng/ml.

引用

页码：251 / 258

页数：8

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