THE GLUCOSINOLATE-DEGRADING ENZYME MYROSINASE IN BRASSICACEAE IS ENCODED BY A GENE FAMILY

被引：95

作者：

XUE, JP

LENMAN, M

FALK, A

RASK, L

机构：

[1] Department of Cell Research, Swedish University of Agricultural Sciences, Uppsala Genetic Center, Uppsala, S-75007

来源：

PLANT MOLECULAR BIOLOGY | 1992年 / 18卷 / 02期

关键词：

ARABIDOPSIS-THALIANA; BRASSICACEAE; BRASSICA-NAPUS; GLUCOSINOLATE; MYROSINASE; SINAPIS-ALBA;

D O I：

10.1007/BF00034965

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A full-length cDNA clone (MB3) and three partial clones (MA1, MB1 and MB2) which encode myrosinase (thioglucoside glucohydrolase, EC 3.2.3.1) were isolated from a Sinapis alba (white mustard) cDNA library. Nucleotide sequence analysis of these clones revealed that they are encoded by a gene family. Southern blot analysis with gene-specific probes showed that the gene family consists of a least two subfamilies (MA and MB) each with several members both in S. alba and in Brassica napus (oilseed rape). In Arabidopsis thaliana (wall cress) only three myrosinase genes seem to be present. Northern blot analysis indicated that all the myrosinase mRNA species have the same size, approximately 1.95 kb.

引用

页码：387 / 398

页数：12

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