MS-282A AND MS-282B, NEW INHIBITORS OF CALMODULIN-ACTIVATED MYOSIN LIGHT-CHAIN KINASE FROM STREPTOMYCES-TAURICUS ATCC-27470

被引：15

作者：

NAKANISHI, S ^{[1
]}

KITA, K ^{[1
]}

UOSAKI, Y ^{[1
]}

YOSHIDA, M ^{[1
]}

SAITOH, Y ^{[1
]}

MIHARA, A ^{[1
]}

KAWAMOTO, I ^{[1
]}

MATSUDA, Y ^{[1
]}

机构：

[1] KYOWA HAKKO KOGYO CO LTD,PHARMACEUT RES LABS,NAGAIZUMI,SHIZUOKA 411,JAPAN

来源：

JOURNAL OF ANTIBIOTICS | 1994年 / 47卷 / 08期

关键词：

D O I：

10.7164/antibiotics.47.855

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

MS-282a and MS-282b were isolated from the culture broth of Streptomyces tala icus ATCC 27470 as inhibitors of smooth muscle myosin light chain kinase (MLCK). MS-282a and MS-282b inhibited the activity of chicken gizzard MLCK with IC50 values of 3.8 mu M and 5.2 mu M, respectively. Cyclic AMP-dependent protein kinase, cyclic GMP-dependent protein kinase and protein kinase C were not inhibited by 150 mu M MS-282a at all. It is likely that MS-282a blocks MLCK activity by antagonizing calmodulin since 1) the compound inhibited calmodulin-dependent but not calmodulin-independent activity of MLCK; 2) the inhibition of MLCK was antagonized by increasing concentrations of calmodulin, and 3) the compound inhibited calmodulin-dependent cyclic nucleotide phosphodiesterase.

引用

页码：855 / 861

页数：7

共 17 条

[1]

ADELSTEIN RS, 1980, CALCIUM CELL FUNCTIO, V1, P161

[2]

Gause GF, 1983, GUIDE DETERMINATION

[3] THYROID-HORMONES INHIBIT THE CA-2+ CALMODULIN-INDUCED ACTIVATION OF MYOSIN LIGHT CHAIN KINASE [J].

HAGIWARA, M ;

MAMIYA, S ;

OCHIAI, M ;

HIDAKA, H .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1988, 152 (01) :270-276

[4] K-252 COMPOUNDS, NOVEL AND POTENT INHIBITORS OF PROTEIN-KINASE-C AND CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN-KINASES [J].

KASE, H ;

IWAHASHI, K ;

NAKANISHI, S ;

MATSUDA, Y ;

YAMADA, K ;

TAKAHASHI, M ;

MURAKATA, C ;

SATO, A ;

KANEKO, M .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1987, 142 (02) :436-440

[5] ISOLATION, PHYSICOCHEMICAL PROPERTIES AND BIOLOGICAL-ACTIVITY OF PAMAMYCIN-607, AN AERIAL MYCELIUM-INDUCING SUBSTANCE FROM STREPTOMYCES-ALBONIGER [J].

KONDO, S ;

YASUI, K ;

NATSUME, M ;

KATAYAMA, M ;

MARUMO, S .

JOURNAL OF ANTIBIOTICS, 1988, 41 (09) :1196-1204

[6] PAMAMYCIN - NEW ANTIBIOTIC AND STIMULATOR OF AERIAL MYCELIA FORMATION [J].

MCCANN, PA ;

POGELL, BM .

JOURNAL OF ANTIBIOTICS, 1979, 32 (07) :673-678

[7]

NAKANISHI S, 1988, J BIOL CHEM, V263, P6215

[8] ASSAY OF MYOSIN LIGHT CHAIN KINASE-ACTIVITY BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY USING A SYNTHETIC PEPTIDE AS SUBSTRATE [J].

NAKANISHI, S ;

KASE, H ;

MATSUDA, Y .

ANALYTICAL BIOCHEMISTRY, 1991, 195 (02) :313-318

[9] KS-505A, A NOVEL INHIBITOR OF BOVINE BRAIN CA2+ AND CALMODULIN-DEPENDENT CYCLIC-NUCLEOTIDE PHOSPHODIESTERASE FROM STREPTOMYCES-ARGENTEOLUS [J].

NAKANISHI, S ;

OSAWA, K ;

SAITO, Y ;

KAWAMOTO, I ;

KURODA, K ;

KASE, H .

JOURNAL OF ANTIBIOTICS, 1992, 45 (03) :341-347

[10] MS-347A, A NEW INHIBITOR OF MYOSIN LIGHT-CHAIN KINASE FROM ASPERGILLUS SP KY52178 [J].

NAKANISHI, S ;

ANDO, K ;

KAWAMOTO, I ;

MATSUDA, Y .

JOURNAL OF ANTIBIOTICS, 1993, 46 (12) :1775-1781

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