CLONING AND SEQUENCE DETERMINATION OF THE SCHIZOSACCHAROMYCES-POMBE RPB2 GENE ENCODING THE SUBUNIT-2 OF RNA POLYMERASE-II

被引：25

作者：

KAWAGISHI, M

YAMAGISHI, M

ISHIHAMA, A

机构：

[1] NATL INST GENET,DEPT MOLEC GENET,MISHIMA,SHIZUOKA 411,JAPAN

[2] UNIV TOKYO,FAC SCI,DEPT BIOPHYS & BIOCHEM,TOKYO 113,JAPAN

来源：

NUCLEIC ACIDS RESEARCH | 1993年 / 21卷 / 03期

关键词：

D O I：

10.1093/nar/21.3.469

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The gene, rpb2, encoding the second largest subunit, subunit 2, of RNA polymerase II has been cloned from Schizosaccharomyces pombe using the corresponding gene, RPB2, of Saccharomyces cerevisiae as a probe for cross-hybridization. We have determined the complete nucleotide sequence of rpb2, and parts of the PCR-amplified rpb2 cDNA. The predicted coding sequence of a polypeptide of 1210 amino acid residues with a calculated molecular weight of 138 kilodaltons was interrupted by a short intron. The overall amino acid sequence homology of the S.pombe subunit 2 is 68, 62 and 62% with the corresponding protein from S.cerevisiae, D.melanogaster and H.sapiens, respectively. Southern analysis of the genomic DNA digested with various restriction enzymes showed that rpb2 was present as a single copy in the S.pombe genome. Northern analysis showed that the transcript of rpb2 was about 4 kb in length.

引用

页码：469 / 473

页数：5

共 29 条

[1] PRIMARY STRUCTURE OF THE 2ND LARGEST SUBUNIT OF HUMAN RNA POLYMERASE-II (OR B) [J].