DISULFIDE BOND-CLEAVAGE OF HUMAN SERUM-ALBUMIN AND ALTERATIONS OF ITS SECONDARY STRUCTURE BY CIS-DIAMMINEDICHLOROPLATINUM(II)

被引：18

作者：

OHTA, N ^{[1
]}

YOTSUYANAGI, T ^{[1
]}

CHEN, D ^{[1
]}

ONO, R ^{[1
]}

ITO, S ^{[1
]}

IKEDA, K ^{[1
]}

机构：

[1] NAGOYA CITY UNIV,FAC PHARMACEUT SCI,NAGOYA,AICHI 467,JAPAN

来源：

INTERNATIONAL JOURNAL OF PHARMACEUTICS | 1992年 / 85卷 / 1-3期

关键词：

CIS-DIAMMINEDICHLOROPLATINUM(II); HUMAN SERUM ALBUMIN; DISULFIDE BOND; SECONDARY STRUCTURE; CIRCULAR DICHROISM; ALPHA-HELIX;

D O I：

10.1016/0378-5173(92)90131-K

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

cis-Diamminedichloroplatinum(II) (cis-DDP) cleaved disulfide (S-S) bonds in human serum albumin (HSA) and brought about alterations of the secondary structure. The alpha-helix content decreased from 50.5% (native) to approx. 33% (four S-S bonds cleaved). The tendency toward a decrease corresponded only with an increase in the beta-sheet. Sulfitolysis of the S-S bonds showed a tendency similar to that of metal binding. Fluorescence and UV difference spectra changed as a function of S-S bond cleavage and led to considerable differences between the two cleaving agents.

引用

页码：39 / 44

页数：6

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