ISOLATION OF 2 GENES THAT AFFECT MITOTIC CHROMOSOME TRANSMISSION IN SACCHAROMYCES-CEREVISIAE

被引：91

作者：

MEEKSWAGNER, D ^{[1
]}

WOOD, JS ^{[1
]}

GARVIK, B ^{[1
]}

HARTWELL, LH ^{[1
]}

机构：

[1] UNIV WASHINGTON, DEPT GENET, SEATTLE, WA 98195 USA

来源：

CELL | 1986年 / 44卷 / 01期

关键词：

D O I：

10.1016/0092-8674(86)90484-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two DNA sequences that reduce mitotix fidelity of chromsome transmission have been identified: MIF1 and MIF2. MIF2 is a unique sequence located on the right arm of chromsome XII that stimulates loss and recombination for both chromosomes V and VII when present in a high copy number plasmid. MIF1 is not essential for cell division but is necessary for the normal fidelity of chromosome transmission. MIF2 is a unique sequence located 15 cM distal to HIS6 on chromosome IX that induce a high frequency of chromosme VII loss and a lower frequency of chromosome V loss when present in high copy number; it has no effect on mitotic recombination. Disruption of the genomic MIF2 locus was lethal and cells lacking this function arrested division with a terminal phenotype characteristic of a block in DNA replication or nuclear division.

引用

页码：53 / 63

页数：11

共 34 条

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HARTWELL LH, 1973, GENETICS, V74, P267

[12] SACCHAROMYCES-CEREVISIAE CELL-CYCLE [J].