SINGLE-MOLECULE DETECTION AS AN APPROACH TO RAPID DNA SEQUENCING

被引：23

作者：

HARDING, JD ^{[1
]}

KELLER, RA ^{[1
]}

机构：

[1] UNIV CALIF LOS ALAMOS SCI LAB,CTR HUMAN GENOME STUDIES,LOS ALAMOS,NM 87545

来源：

TRENDS IN BIOTECHNOLOGY | 1992年 / 10卷 / 1-2期

关键词：

D O I：

10.1016/0167-7799(92)90170-Z

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Current sequencing technologies are insufficient to cope with large-scale projects such as sequencing the human genome and genomes of model organisms. In addition, as genetic lesions associated with specific human diseases are identified, DNA sequencing will be used increasingly for clinical applications. Thus, new approaches are needed to combine high-throughput with accuracy for both research and diagnostic purposes. A novel technology based on detection of individual fluorescent nucleotides in a flowing sample stream is under development.

引用

页码：55 / 57

页数：3

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