COMPARISON OF THE NUMBER OF IL-4 AND IFN-GAMMA SECRETING CELLS IN RESPONSE TO THE MALARIA VACCINE CANDIDATE ANTIGEN PF155/RESA IN 2 GROUPS OF NATURALLY PRIMED INDIVIDUALS LIVING IN A MALARIA ENDEMIC AREA IN BURKINA-FASO

被引:16
作者
ELGHAZALI, G
ESPOSITO, F
TROYEBLOMBERG, M
机构
[1] UNIV CAMERINO, DEPT BIOL, I-62032 CAMERINO, ITALY
[2] CTR LUTTE CONTRE PALUDISME, OUAGADOUGOU, BURKINA FASO
关键词
D O I
10.1111/j.1365-3083.1995.tb03623.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The enzyme-linked immunospot (ELISPOT) assay was used to enumerate the number of IFN-gamma and IL-4 producing cells after in vitro stimulation with a highly purified recombinant malaria vaccine candidate antigen (r-Pf155/RESA) or synthetic peptides corresponding to its major T-cell epitopes. Two groups of naturally primed individuals living in rural areas of Burkina Faso were studied. The donors comprised one group of healthy (non-parasitemic) mainly adult people and one parasitemic mainly younger people. IL-4 producing cells were detected in response to PHA but no such cells were detected in response to the malarial antigens. The most frequent IFN-gamma responses were seen with r-Pf155/RESA. Thus, after stimulation with this antigen 52% of the donors responded positively in the ELISPOT assay, while only 17% responded to the synthetic peptides, suggesting that the r-Pf155/RESA contained T-cell epitopes not covered by the peptides used in this study. The number of IFN-gamma producing cells in response to the malarial antigens did not differ between the two groups. However, IFN-gamma levels found in sera from the parasitemic individuals were significantly higher than in those from healthy donors. This latter finding and the lack of differences seen in the number of IFN-gamma producing spots in the two groups indicate that IFN-gamma producing cells may have sequestered to other organs in the parasitemic group.
引用
收藏
页码:39 / 45
页数:7
相关论文
共 39 条
[1]   PF155/RESA ANTIGEN IS LOCALIZED IN DENSE GRANULES OF PLASMODIUM-FALCIPARUM MEROZOITES [J].
AIKAWA, M ;
TORII, M ;
SJOLANDER, A ;
BERZINS, K ;
PERLMANN, P ;
MILLER, LH .
EXPERIMENTAL PARASITOLOGY, 1990, 71 (03) :326-329
[2]  
ALPERS MP, 1992, PAPUA NEW GUINEA MED, V35, P285
[3]   MONOCLONAL-ANTIBODY 2-SITE ELISA FOR HUMAN IFN-GAMMA - ADAPTATION FOR DETERMINATIONS IN HUMAN-SERUM OR PLASMA [J].
ANDERSSON, G ;
EKRE, HPT ;
ALM, G ;
PERLMANN, P .
JOURNAL OF IMMUNOLOGICAL METHODS, 1989, 125 (1-2) :89-96
[4]  
CHOUGNET C, 1991, J IMMUNOL, V147, P2295
[5]   IMMUNE SERA RECOGNIZE ON ERYTHROCYTES A PLASMODIUM-FALCIPARUM ANTIGEN COMPOSED OF REPEATED AMINO-ACID-SEQUENCES [J].
COPPEL, RL ;
COWMAN, AF ;
ANDERS, RF ;
BIANCO, AE ;
SAINT, RB ;
LINGELBACH, KR ;
KEMP, DJ ;
BROWN, GV .
NATURE, 1984, 310 (5980) :789-792
[6]   PROTECTIVE VALUE OF ELEVATED LEVELS OF GAMMA-INTERFERON IN SERUM AGAINST EXOERYTHROCYTIC STAGES OF PLASMODIUM-FALCIPARUM [J].
DELORON, P ;
CHOUGNET, C ;
LEPERS, JP ;
TALLET, S ;
COULANGES, P .
JOURNAL OF CLINICAL MICROBIOLOGY, 1991, 29 (09) :1757-1760
[7]   PLASMODIUM-FALCIPARUM-SPECIFIC IMMUNOGLOBULIN-G (IGG), IGM, AND IGE ANTIBODIES IN PAIRED MATERNAL-CORD SERA FROM EAST SEPIK PROVINCE, PAPUA-NEW-GUINEA [J].
DESOWITZ, RS ;
ELM, J ;
ALPERS, MP .
INFECTION AND IMMUNITY, 1993, 61 (03) :988-993
[8]   PLASMODIUM-SPECIFIC IMMUNOGLOBULIN-E IN SERA FROM AN AREA OF HOLOENDEMIC MALARIA [J].
DESOWITZ, RS .
TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 1989, 83 (04) :478-479
[9]   REGULATION OF IGE SYNTHESIS BY CYTOKINES [J].
DEVRIES, JE ;
GAUCHAT, JF ;
AVERSA, GG ;
PUNNONEN, J ;
GASCAN, H ;
YSSEL, H .
CURRENT OPINION IN IMMUNOLOGY, 1991, 3 (06) :851-858
[10]   NUMBER OF INTERLEUKIN-4-SECRETING AND INTERFERON-GAMMA-SECRETING HUMAN T-CELLS REACTIVE WITH TETANUS TOXOID AND THE MYCOBACTERIAL ANTIGEN PPD OR PHYTOHEMAGGLUTININ - DISTINCT RESPONSE PROFILES DEPENDING ON THE TYPE OF ANTIGEN USED FOR ACTIVATION [J].
ELGHAZALI, GEB ;
PAULIE, S ;
ANDERSSON, G ;
HANSSON, Y ;
HOLMQUIST, G ;
SUN, JB ;
OLSSON, T ;
EKRE, HP ;
TROYEBLOMBERG, M .
EUROPEAN JOURNAL OF IMMUNOLOGY, 1993, 23 (11) :2740-2745