TRANSCRIPTION FACTOR DNA-BINDING ACTIVITY IN PC12 CELLS UNDERGOING APOPTOSIS AFTER GLUCOSE DEPRIVATION

被引：43

作者：

TONG, LQ ^{[1
]}

PEREZPOLO, JR ^{[1
]}

机构：

[1] UNIV TEXAS,MED BRANCH,DEPT HUMAN BIOL CHEM & GENET,GALVESTON,TX 77555

来源：

NEUROSCIENCE LETTERS | 1995年 / 191卷 / 03期

关键词：

PC12; CELL DEATH; TRANSCRIPTION FACTOR; APOPTOSIS; GLUCOSE DEPRIVATION; NERVE GROWTH FACTOR;

D O I：

10.1016/0304-3940(95)11557-D

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Following hypoglycemic injury to rat pheochromocytoma PC12 cells, cells display nuclear chromatin condensation and nucleosome-sized DNA fragmentation. Electrophoretic mobility shift assays were used to characterize binding of nuclear proteins to consensus sequences for AP-1, nuclear factor kappa B (NF kappa B), and octamer family after glucose deprivation. While AP-1 DNA binding activity and NF kappa B DNA binding activity were transiently stimulated, DNA binding to the octamer motif decreased. These data suggest that changes in nuclear protein binding to specific consensus sequences are an early molecular event in hypoglycemic-ischemic injury-induced neuronal cell death.

引用

页码：137 / 140

页数：4

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