A COMPARISON OF 2 METHODS FOR ISOLATION OF SALMONELLA FROM POULTRY LITTER SAMPLES

被引：23

作者：

READ, SC

IRWIN, RJ

POPPE, C

HARRIS, J

机构：

[1] Agriculture and Agri-Food, Health of Animals Laboratory, Guelph, Ontario

来源：

POULTRY SCIENCE | 1994年 / 73卷 / 10期

关键词：

SALMONELLA; DETECTION; POULTRY LITTER; MODIFIED SEMISOLID RAPPAPORT VASSILIADIS AGAR; XYLOSE LYSINE TERGITOL-4 AGAR;

D O I：

10.3382/ps.0731617

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Two methods were compared to determine their ability and efficiency in detecting Salmonella in poultry litter samples. Method 1 consisted of pre-enrichment in buffered peptone water (BPW), selective motility enrichment in Modified Semisolid Rappaport Vassiliadis (MSRV) agar, and plating onto MacConkey (MC) agar. Method 2 employed tetrathionate brilliant green (TBG) broth and plating on brilliant green agar with novobiocin (BGAN) and on xylose lysine tergitol 4 (XLT4) agar. Method 1 resulted in a significantly higher isolation rate, was cheaper, and was less labor intensive.

引用

页码：1617 / 1621

页数：5

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