ATOMIC FORCE MICROSCOPY OF BIOLOGICAL SAMPLES AT LOW-TEMPERATURE

被引：50

作者：

PRATER, CB ^{[1
]}

WILSON, MR ^{[1
]}

GARNAES, J ^{[1
]}

MASSIE, J ^{[1
]}

ELINGS, VB ^{[1
]}

HANSMA, PK ^{[1
]}

机构：

[1] DIGITAL INSTRUMENTS,SANTA BARBARA,CA 93117

来源：

JOURNAL OF VACUUM SCIENCE & TECHNOLOGY B | 1991年 / 9卷 / 02期

关键词：

D O I：

10.1116/1.585442

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

The atomic force microscope (AFM) has been successful imaging rigid samples with atomic resolution, but this resolution has not been matched on soft biological samples at room temperature. On many biological samples even tracking forces as small as 10(-9) N distort the sample during a scan. At low temperatures proteins freeze into a single conformational state and become much more rigid. In this paper we demonstrate that it should be feasible to get atomic resolution on frozen biological samples with a low-temperature AFM. We also show some preliminary low-temperature AFM images of one biological sample, purple membrane.

引用

页码：989 / 991

页数：3

共 21 条

[1] ATOMIC RESOLUTION IMAGING OF A NONCONDUCTOR BY ATOMIC FORCE MICROSCOPY [J].

ALBRECHT, TR ;

QUATE, CF .

JOURNAL OF APPLIED PHYSICS, 1987, 62 (07) :2599-2602

[2] ATOMIC FORCE MICROSCOPE [J].

BINNIG, G ;

QUATE, CF ;

GERBER, C .

PHYSICAL REVIEW LETTERS, 1986, 56 (09) :930-933

[3]

CHIU W, 1986, ANNU REV BIOPHYS BIO, V15, P237

[4]

DORRINGTON KL, 1979, MECHANICAL PROPERTIE

[5] IMAGING CRYSTALS, POLYMERS, AND PROCESSES IN WATER WITH THE ATOMIC FORCE MICROSCOPE [J].

DRAKE, B ;

PRATER, CB ;

WEISENHORN, AL ;

GOULD, SAC ;

ALBRECHT, TR ;

QUATE, CF ;

CANNELL, DS ;

HANSMA, HG ;

HANSMA, PK .

SCIENCE, 1989, 243 (4898) :1586-1589

[6] FROM ATOMS TO INTEGRATED-CIRCUIT CHIPS, BLOOD-CELLS, AND BACTERIA WITH THE ATOMIC FORCE MICROSCOPE [J].

GOULD, SAC ;

DRAKE, B ;

PRATER, CB ;

WEISENHORN, AL ;

MANNE, S ;

HANSMA, HG ;

HANSMA, PK ;

MASSIE, J ;

LONGMIRE, M ;

ELINGS, V ;

NORTHERN, BD ;

MUKERGEE, B ;

PETERSON, CM ;

STOECKENIUS, W ;

ALBRECHT, TR ;

QUATE, CF .

JOURNAL OF VACUUM SCIENCE & TECHNOLOGY A-VACUUM SURFACES AND FILMS, 1990, 8 (01) :369-373

[7]

GOULD SAC, 1988, NATURE, V32, P332

[8] GLASSY BEHAVIOR OF A PROTEIN [J].

IBEN, IET ;

BRAUNSTEIN, D ;

DOSTER, W ;

FRAUENFELDER, H ;

HONG, MK ;

JOHNSON, JB ;

LUCK, S ;

ORMOS, P ;

SCHULTE, A ;

STEINBACH, PJ ;

XIE, AH ;

YOUNG, RD .

PHYSICAL REVIEW LETTERS, 1989, 62 (16) :1916-1919

[9] LOW-TEMPERATURE ATOMIC FORCE MICROSCOPY [J].

KIRK, MD ;

ALBRECHT, TR ;

QUATE, CF .

REVIEW OF SCIENTIFIC INSTRUMENTS, 1988, 59 (06) :833-835

[10] IMAGING METAL ATOMS IN AIR AND WATER USING THE ATOMIC FORCE MICROSCOPE [J].

MANNE, S ;

BUTT, HJ ;

GOULD, SAC ;

HANSMA, PK .

APPLIED PHYSICS LETTERS, 1990, 56 (18) :1758-1759

← 1 2 3 →