EFFECT OF PROTEOLYTIC-ENZYMES ON THE VITAMIN-B12-BINDING PROTEINS OF HUMAN GASTRIC-JUICE AND SALIVA

被引:17
作者
ANDERSEN, KJ
VONDERLIPPE, G
机构
[1] Section for Clinical Research and Molecular Medicine, Medical Dept. A, University of Bergen, Haukeland Sykehus, Bergen
关键词
Brush border; Gastric juice; Gel filtration; Lysosomal enzymes; Pancreatic disease; Pepsin; Saliva; Trypsin;
D O I
10.3109/00365527909181412
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Pepsin had no effect on the vitamin B12 binder in human saliva (R-binder), while trypsin was found to reduce the apparent molecular weight of the R-binder and to release vitamin B12 from the R-B12 complex of human saliva and human gastric juice (HGJ). Trypsin had no effect on the molecular weight and biological activity of intrinsic factor (IF) in HGJ, as demonstrated by gel filtration on Sephadex G-150 and the uptake of IF-B12 by guinea pig intestinal brush borders. An extract of purified guinea pig intestinal lysosomes was also without effect on the molecular weight and the biological activity of IF but was found to release vitamin B12 from the R-B12 complex. The results support the observation that the external pancreatic secretion corrects malabsorption of vitamin B12 by an effect on the non-IF protein in the intestinal juice. Moreover, the results indicate that lysosomal enzymes are not involved in the intestinal absorption of vitamin B12. © 1979 Informa UK Ltd.
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页码:833 / 838
页数:6
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