INDIRECT DETECTION IN CAPILLARY ELECTROPHORESIS - COMPARISON BETWEEN INDIRECT UV AND INDIRECT LASER-INDUCED FLUORESCENCE DETECTION FOR THE DETERMINATION OF ISOPRENYL PYROPHOSPHATES

被引：21

作者：

ANDERSSON, PE ^{[1
]}

PFEFFER, WD ^{[1
]}

BLOMBERG, LG ^{[1
]}

机构：

[1] UNIV STOCKHOLM,ARRHENIUS LABS NAT SCI,DEPT ANALYT CHEM,S-10691 STOCKHOLM,SWEDEN

来源：

JOURNAL OF CHROMATOGRAPHY A | 1995年 / 699卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(95)00019-J

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Three different detection modes were compared for the determination of isoprenyl pyrophosphates by capillary electrophoresis, viz., direct UV, indirect UV and indirect laser-induced fluorescence (ILIF). The composition of the background electrolyte was varied accordingly. On the basis of separation efficiency (N = 600 000) and limit of detection (ca. 0.5 mu M), the ILIF method gave the best results. Separation was complete in less than 6 min.

引用

页码：323 / 330

页数：8

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