INDIRECT DETECTION IN CAPILLARY ELECTROPHORESIS - COMPARISON BETWEEN INDIRECT UV AND INDIRECT LASER-INDUCED FLUORESCENCE DETECTION FOR THE DETERMINATION OF ISOPRENYL PYROPHOSPHATES

被引：21

作者：

ANDERSSON, PE ^{[1
]}

PFEFFER, WD ^{[1
]}

BLOMBERG, LG ^{[1
]}

机构：

[1] UNIV STOCKHOLM,ARRHENIUS LABS NAT SCI,DEPT ANALYT CHEM,S-10691 STOCKHOLM,SWEDEN

来源：

JOURNAL OF CHROMATOGRAPHY A | 1995年 / 699卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(95)00019-J

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Three different detection modes were compared for the determination of isoprenyl pyrophosphates by capillary electrophoresis, viz., direct UV, indirect UV and indirect laser-induced fluorescence (ILIF). The composition of the background electrolyte was varied accordingly. On the basis of separation efficiency (N = 600 000) and limit of detection (ca. 0.5 mu M), the ILIF method gave the best results. Separation was complete in less than 6 min.

引用

页码：323 / 330

页数：8

共 42 条

[31] INDIRECT UV PHOTOMETRIC DETECTION IN CAPILLARY ZONE ELECTROPHORESIS FOR THE DETERMINATION OF PHYTATE IN SOYBEANS [J].

NARDI, A ;

CRISTALLI, M ;

DESIDERIO, C ;

OSSICINI, L ;

SHUKLA, SK ;

FANALI, S .

JOURNAL OF MICROCOLUMN SEPARATIONS, 1992, 4 (01) :9-11

[32] QUANTITATIVE ASPECTS OF INDIRECT UV DETECTION IN CAPILLARY ZONE ELECTROPHORESIS [J].

NIELEN, MWF .

JOURNAL OF CHROMATOGRAPHY, 1991, 588 (1-2) :321-326

[33]

POPJAK G, 1962, J BIOL CHEM, V237, P56

[34] ENSEMBLE AVERAGING AND DIGITAL FILTERING IN CHROMATOGRAPHY AND ELECTROPHORESIS [J].

REGEHR, MF ;

PALIWAL, SK ;

REGNIER, FE .

JOURNAL OF CHROMATOGRAPHY A, 1994, 659 (02) :247-253

[35] PRENYLATED PROTEINS - THE STRUCTURE OF THE ISOPRENOID GROUP [J].

RILLING, HC ;

BREUNGER, E ;

EPSTEIN, WW ;

CRAIN, PF .

SCIENCE, 1990, 247 (4940) :318-320

[36] DEFECTIVE MACROMOLECULE BIOSYNTHESIS AND CELL-CYCLE PROGRESSION IN A MAMMALIAN-CELL STARVED FOR MEVALONATE [J].

SINENSKY, M ;

LOGEL, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (10) :3257-3261

[37] EFFECT OF PHOTOBLEACHING ON THE OUTPUT OF AN ON-COLUMN LASER FLUORESCENCE DETECTOR [J].

SUGARMAN, JH ;

PRUDHOMME, RK .

INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH, 1987, 26 (07) :1449-1454

[38] NOISE AND DETECTION LIMITS OF INDIRECT ABSORPTION DETECTION IN CAPILLARY ZONE ELECTROPHORESIS [J].

WANG, TS ;

HARTWICK, RA .

JOURNAL OF CHROMATOGRAPHY, 1992, 607 (01) :119-125

[39] ANALYSIS OF ANION CONSTITUENTS OF URINE BY INORGANIC CAPILLARY ELECTROPHORESIS [J].

WILDMAN, BJ ;

JACKSON, PE ;

JONES, WR ;

ALDEN, PG .

JOURNAL OF CHROMATOGRAPHY, 1991, 546 (1-2) :459-466

[40] INDIRECT DETECTION METHODS - LOOKING FOR WHAT IS NOT THERE [J].

YEUNG, ES .

ACCOUNTS OF CHEMICAL RESEARCH, 1989, 22 (04) :125-130

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