H-1 NMR-STUDY OF CONVERSION OF 13(S)-HYDROPEROXY LINOLEIC-ACID BY SOYA BEAN LIPOXYGENASE-I

被引：5

作者：

EGMOND, MR

WILLIAMS, RJP

机构：

[1] Inorganic Chemistry Laboratory, Oxford, 0X1 3QR, South Parks Road

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1978年 / 531卷 / 02期

关键词：

D O I：

10.1016/0005-2760(78)90137-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The conversion of 13(S)-hydroperoxy linoleic acid by lipoxygenase I at 298 K was monitored by 1H NMR and ultraviolet absorption spectroscopy. The rate constant for the conversion of the hydroperoxide, k = 45.8 ± 7.5 M-1 · s-1, depends on the concentrations of both enzyme and hydroperoxide. This constant is not affected by O2, nor by solvent isotope effects. © 1978.

引用

页码：141 / 148

页数：8

共 24 条

[1] AOSHIMA H, 1977, BIOCHIM BIOPHYS ACTA, V486, P121
[2] PULSED NMR METHODS FOR OBSERVATION AND ASSIGNMENT OF EXCHANGEABLE HYDROGENS - APPLICATION TO BACITRACIN
CAMPBELL, ID
DOBSON, CM
JEMINET, G
WILLIAMS, RJP
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[3] THERMAL-DECOMPOSITION OF INDIVIDUAL POSITIONAL ISOMERS OF METHYL LINOLEATE HYDROPEROXIDE - EVIDENCE OF CARBON-OXYGEN BOND SCISSION
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[J]. JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1976, 53 (09) : 572 - 576
[4] SOYBEAN LIPOXYGENASE - IRON-CONTAINING DIOXYGENASE
CHAN, HWS
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1973, 327 (01) : 32 - 35
[5] DEMONSTRATION BY EPR SPECTROSCOPY OF FUNCTIONAL ROLE OF IRON IN SOYBEAN LIPOXYGENASE-1
DEGROOT, JJM
VELDINK, GA
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BOLDINGH, J
WEVER, R
VANGELDER, BF
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1975, 377 (01) : 71 - 79
[6] DETECTION OF LINOLEIC-ACID RADICALS IN ANAEROBIC REACTION OF LIPOXYGENASE
DEGROOT, JJM
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VLIEGENTHART, JF
BOLDINGH, J
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1973, 326 (02) : 279 - 284
[7] INTERACTION OF SOYBEAN LIPOXYGENASE-1 AND 13-L-HYDROPEROXYLINOLEIC ACID, INVOLVING YELLOW AND PURPLE COLORED ENZYME SPECIES
DEGROOT, JJM
GARSSEN, GJ
VELDINK, GA
VLIEGENTHART, JFG
BOLDINGH, J
EGMOND, MR
[J]. FEBS LETTERS, 1975, 56 (01) : 50 - 54
[8] MECHANISM OF ACTION OF SOYBEAN LIPOXYGENASE-1 - STOPPED-FLOW KINETIC STUDY OF FORMATION AND CONVERSION OF YELLOW AND PURPLE ENZYME SPECIES
EGMOND, MR
FASELLA, PM
VELDINK, GA
VLIEGENTHART, JFG
BOLDINGH, J
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1977, 76 (02): : 469 - 479
[9] CHANGES IN FLUORESCENCE AND ABSORBANCE OF LIPOXYGENASE-1 INDUCED BY 13-LS-HYDROPEROXYLINOLEIC ACID AND LINOLEIC-ACID
EGMOND, MR
FINAZZIAGRO, A
FASELLA, PM
VELDINK, GA
VLIEGENTHART, JFG
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1975, 397 (01) : 43 - 49
[10] INFLUENCE OF OXYGEN ON FLUORESCENCE OF LIPOXYGENASE
FINAZZIAGRO, A
AVIGLIANO, L
VELDINK, GA
VLIEGENTHART, JF
BOLDINGH, J
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1973, 326 (03) : 462 - 470

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