Maleate cis-trans isomerase from Arthrobacter sp TPU 5446

被引：14

作者：

Kato, Y ^{[1
]}

Yamagishi, J ^{[1
]}

Asano, Y ^{[1
]}

机构：

[1] TOYAMA PREFECTURAL UNIV,FAC ENGN,BIOTECHNOL RES CTR,KOSUGI,TOYAMA 93903,JAPAN

来源：

JOURNAL OF FERMENTATION AND BIOENGINEERING | 1995年 / 80卷 / 06期

关键词：

maleate cis-trans isomerase; enzymatic isomerization; Arthrobacter sp;

D O I：

10.1016/0922-338X(96)87741-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

gram-positive aerobic bacterium, Arthrobacter sp. strain TPU 5446 with a high maleate cis-trans isomerase (MAI) activity, was isolated from soil. Enzyme activity was induced when the strain was cultivated in the presence of maleic acid and yeast extract as the carbon and nitrogen sources, respectively. From a crude extract of the cells, the enzyme was partially purified by ammonium sulfate fractionation and DEAE-Toyopearl column chromatography. The enzyme acted specifically on maleic acid and produced fumaric acid stoichiometrically.

引用

页码：610 / 612

页数：3

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