FTMS METHOD FOR HIGH-RESOLUTION MATRIX-ASSISTED LASER-DESORPTION

被引：38

作者：

MCIVER, RT ^{[1
]}

LI, YZ ^{[1
]}

HUNTER, RL ^{[1
]}

机构：

[1] IONSPEC CORP,IRVINE,CA 92714

来源：

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY AND ION PROCESSES | 1994年 / 132卷 / 03期

关键词：

MATRIX-ASSISTED LASER DESORPTION-IONIZATION; LASER DESORPTION; FOURIER TRANSFORM MASS SPECTROMETRY; INSULIN; GRAMICIDIN-S;

D O I：

10.1016/0168-1176(94)85003-8

中图分类号：

O64 [物理化学（理论化学）、化学物理学]; O56 [分子物理学、原子物理学];

学科分类号：

070203 ; 070304 ; 081704 ; 1406 ;

摘要：

A Fourier transform mass spectrometer with an external ion source has been modified for use with matrix-assisted laser desorption. High trapping potentials on the FTMS analyzer cell decelerate and trap the laser-produced ions, and a pulsed argon buffer gas cools them prior to detection. For gramicidin S, only one laser pulse is needed to produce mass spectra with a high signal-to-noise ratio and a mass resolution of 1 100 000 (FWHM). Several other oligopeptides and small proteins have been analyzed, including bovine insulin that was detected at a mass resolution of 90 000. These results represent the highest mass resolution ever demonstrated for ions made by MALDI.

引用

页码：L1 / L7

页数：7

共 31 条

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