RFLP MARKERS LINKED TO POWDERY MILDEW RESISTANCE GENES PM1, PM2, PM3, AND PM4 IN WHEAT

被引：122

作者：

MA, ZQ ^{[1
]}

SORRELLS, ME ^{[1
]}

TANKSLEY, SD ^{[1
]}

机构：

[1] CORNELL UNIV, DEPT PLANT BREEDING & BIOMETRY, ITHACA, NY 14853 USA

来源：

GENOME | 1994年 / 37卷 / 05期

关键词：

RFLP MARKERS; PM1; PM2; PM3; PM4; BLUMERIA GRAMINIS (DC) EO SPEER F SP TRITICI (ERYSIPHE GRAMINIS F SP TRITICI); WHEAT (TRITICUM AESTIVUM L EM THELL); GENE TAGGING;

D O I：

10.1139/g94-123

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Near-isogenic lines (NILs) and their recurrent parent Chancellor (Cc) were used to identify restriction fragment length polymorphic markers linked to powdery mildew (Blumeria graminis (DC.) E.O. Speer f.sp. tritici) resistance genes Pm1, Pm2, Pm3, and Pm4 in wheat (Triticum aestivum L. em. Thell). By mapping these polymorphic markers in F-2 progenies from crosses of the NILs with Cc, it was found that Pm1 cosegregated with a polymorphic locus detected by DNA probe CDO347; Pm2 was linked to a locus detected by probe BCD1871 with a distance of 3.5 cM; Pm3b was linked to a locus detected by probe BCD1434 with a distance of 1.3 cM; Pm4a cosegregated with Xbcd1231-2A(2) and Xcdo678-2A, and was closely flanked by Xbcd1231-2A(1) and Xbcd292-2A both with a distance of 1.5 cM. Aneuploid mapping of these markers indicated that locus Xcdo347-7A is on 7AL, Xbcd1871-5D on 5DS, Xbcd1434-1A on 1AS, and loci Xbcd292-2A and Xcdo678-2A are on 2AL. The same polymorphic fragments detected in the Pm3b NIL by Xbcd1434-1A were found in Pm3a NIL using several enzyme digestions

引用

页码：871 / 875

页数：5

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