支气管哮喘不同中医证型间Treg细胞/Th17细胞失衡的研究

被引：0

作者：

严璐

机构：

[1] 南京中医药大学

关键词：

支气管哮喘; IL-10; IL-17A; 热哮; 肾阳虚证; Treg细胞/Th17细胞失衡;

D O I：

暂无

年度学位：

2012

学位类型：

硕士

导师：

周贤梅;

摘要：

目的：支气管哮喘中医辨病属“哮病”范畴,现代研究发现,调节性T细胞(Treg细胞)与Th17细胞之间的失衡在支气管哮喘发病机制中起着重要作用。Treg细胞特异性表达白介素(IL).10,而Thl7细胞特异性表达IL-17A。本研究通过测定不同中医证型的支气管哮喘患者血清中IL-10和IL-17A水平,间接反映Treg细胞及Th17细胞失衡情况,从而探讨哮病不同中医证型问的免疫状态,为中医辨证提供客观依据,进而有利于临床诊治。方法：选取支气管哮喘患者30例,其中处于发作期且辨证属热哮患者15例,处于缓解期且辨证属肾阳虚患者15例,及正常志愿者15例。采用不含内毒素及热源的试管采集受试者外周血,4000r/min离心15min分离血清,置于-80℃冰箱保存,采用酶联免疫吸附法(ELISA)试剂盒测定血清IL-10和IL-17A水平。结果：1、与正常组血清IL-10水平(104.22±42.07)比较,热哮组(64.76±8.18)、肾阳虚组(57.62±12.94)均显著降低(P<0.05)。支气管哮喘两证型组间比较,无统计学差异(P>0.05)。2、热哮组血清IL-17A水平(62.05±13.25)与正常组(50.26±9.84)比较显著升高(P<0.05),肾阳虚组血清IL-17A水平(54.89±13.80)与正常组相比较无统计学差异(P>0.05)。支气管哮喘两证型组间不存在统计学差异(P>0.05)。3、与正常组血清IL-10/IL-17A比值(2.10±0.90)比较,热哮组(1.08±0.20)和肾阳虚组(1.11±0.37)两者比值均显著减低(P<0.05)。哮喘两证型组间比较无统计学差异(P>0.05)。结论：1、哮喘热哮证患者血清IL-10水平降低,IL-17A水平升高,IL-10/IL-17A比值降低,提示热哮证患者存在Treg细胞/Th17细胞失衡,是由于IL-10降低同时IL-17A升高所致。2、哮喘肾阳虚证患者血IL-10水平降低,IL-17A水平正常,IL-10/IL-17A比值降低,提示。肾阳虚证患者存在Treg细胞/Th17细胞失衡,是由于IL-10水平降低所致。3、哮喘热哮证和肾阳虚证均存在一定的免疫状态异常,但血清IL-10/IL-17A或Treg细胞／Th17细胞失衡尚不能作为临床哮喘中医辨证分型的参考指标。

引用

页数：40

共 53 条

[1]

Natural Foxp3<sup>+</sup> regulatory T cells inhibit Th2 polarization but are biased toward suppression of Th17‐driven lung inflammation.[J].Teri Girtsman;Zeina Jaffar;Maria Ferrini;Pamela Shaw;Kevan Roberts.Journal of Leukocyte Biology.2010, 3

[2]

IL-21 regulates Th17 cells in rheumatoid arthritis [J].

Niu, Xiaoyin ;

He, Dongyi ;

Zhang, Xin ;

Yue, Tao ;

Li, Ningli ;

Zhang, Jingwu Z. ;

Dong, Chen ;

Chen, Guangjie .

HUMAN IMMUNOLOGY, 2010, 71 (04) :334-341

[3]

The functional plasticity of T cell subsets [J].

Bluestone, Jeffrey A. ;

Mackay, Charles R. ;

O'Shea, John J. ;

Stockinger, Brigitta .

NATURE REVIEWS IMMUNOLOGY, 2009, 9 (11) :811-816

[4]

Regulatory T Cells Reinforce Intestinal Homeostasis [J].

Barnes, Michael J. ;

Powrie, Fiona .

IMMUNITY, 2009, 31 (03) :401-411

[5]

Functional Delineation and Differentiation Dynamics of Human CD4+ T Cells Expressing the FoxP3 Transcription Factor [J].

Miyara, Makoto ;

Yoshioka, Yumiko ;

Kitoh, Akihiko ;

Shima, Tomoko ;

Wing, Kajsa ;

Niwa, Akira ;

Parizot, Christophe ;

Taflin, Cecile ;

Heike, Toshio ;

Valeyre, Dominique ;

Mathian, Alexis ;

Nakahata, Tatsutoshi ;

Yamaguchi, Tomoyuki ;

Nomura, Takashi ;

Ono, Masahiro ;

Amoura, Zahir ;

Gorochov, Guy ;

Sakaguchi, Shimon .

IMMUNITY, 2009, 30 (06) :899-911

[6]

Differentiation and functional analysis of human TH17 cells [J].

Burgler, Simone ;

Ouaked, Nadia ;

Bassin, Claudio ;

Basinski, Tomasz M. ;

Mantel, Pierre-Yves ;

Siegmund, Kerstin ;

Meyer, Norbert ;

Akdis, Cezmi A. ;

Schmidt-Weber, Carsten B. .

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 2009, 123 (03) :588-595

[7]

Human interleukin 17-producing cells originate from a CD161+CD4+ T cell precursor [J].

Cosmi, Lorenzo ;

De Palma, Raffaele ;

Santarlasci, Veronica ;

Maggi, Laura ;

Capone, Manuela ;

Frosali, Francesca ;

Rodolico, Gabriella ;

Querci, Valentina ;

Abbate, Gianfranco ;

Angeli, Roberta ;

Berrino, Liberato ;

Fambrini, Massimiliano ;

Caproni, Marzia ;

Tonelli, Francesco ;

Lazzeri, Elena ;

Parronchi, Paola ;

Liotta, Francesco ;

Maggi, Enrico ;

Romagnani, Sergio ;

Annunziato, Francesco .

JOURNAL OF EXPERIMENTAL MEDICINE, 2008, 205 (08) :1903-1916

[8]

Foxp3 inhibits RORgammat-mediated IL-17A mRNA transcription through direct interaction with RORgammat..[J].Ichiyama Kenji;Yoshida Hideyuki;Wakabayashi Yu;Chinen Takatoshi;Saeki Kazuko;Nakaya Mako;Takaesu Giichi;Hori Shohei;Yoshimura Akihiko;Kobayashi Takashi.The Journal of biological chemistry.2008, 25

[9]

CD4+CD25+ Regulatory T Cells Suppress Mast Cell Degranulation and Allergic Responses through OX40-OX40L Interaction [J].

Gri, Giorgia ;

Piconese, Silvia ;

Frossi, Barbara ;

Manfroi, Vanessa ;

Merluzzi, Sonia ;

Tripodo, Claudio ;

Viola, Antonella ;

Odom, Sandra ;

Rivera, Juan ;

Colombo, Mario P. ;

Pucillo, Carlo E. .

IMMUNITY, 2008, 29 (05) :771-781

[10]

T helper 17 lineage differentiation is programmed by orphan nuclear receptors RORα and RORγ [J].

Yang, Xuexian O. ;

Pappu, Bhanu P. ;

Nurieva, Roza ;

Akimzhanov, Askar ;

Kang, Hong Soon ;

Chung, Yeonseok ;

Ma, Li ;

Shah, Bhavin ;

Panopoulos, Athanasia D. ;

Schluns, Kimberly S. ;

Watowich, Stephanie S. ;

Tian, Qiang ;

Jetten, Anton M. ;

Dong, Chen .

IMMUNITY, 2008, 28 (01) :29-39

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