IMPROVED DETECTION LIMITS FOR ELECTROSPRAY-IONIZATION ON A MAGNETIC-SECTOR MASS-SPECTROMETER BY USING AN ARRAY DETECTOR

被引：14

作者：

CODY, RB

TAMURA, J

FINCH, JW

MUSSELMAN, BD

机构：

[1] JEOL USA, Inc., Peabody, 01960, MA

来源：

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY | 1994年 / 5卷 / 03期

关键词：

D O I：

10.1016/1044-0305(94)85033-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Array detection was compared with point detection for solutions of hen egg-white lysozyme, equine myoglobin, and ubiquitin analyzed by electrospray ionization with a magnetic sector mass spectrometer. The detection limits for samples analyzed by using the array detector system were at least 10 times lower than could be achieved by using a point detector on the same mass spectrometer. The minimum detectable quantity of protein corresponded to a signal-to-background ratio of approximately 2:1 for a 500 amol/muL solution of hen egg-white lysozyme. However, the ultimate practical sample concentrations appeared to be in the 10-100 fmol/muL range for the analysis of dilute solutions of relatively pure proteins or simple mixtures.

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页码：194 / 200

页数：7

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