PERMEABILIZATION OF ESCHERICHIA-COLI-CELLS FOR ENHANCED PENICILLIN ACYLASE ACTIVITY

被引：16

作者：

NAGALAKSHMI, V ^{[1
]}

PAI, JS ^{[1
]}

机构：

[1] UNIV BOMBAY, DEPT CHEM TECHNOL, BOMBAY 400019, INDIA

来源：

BIOTECHNOLOGY TECHNIQUES | 1994年 / 8卷 / 06期

关键词：

D O I：

10.1007/BF00154316

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Escherichia coli cells with penicillin acylase activity were permeabilized with aqueous solutions of the cationic detergent N-cetyl-N,N,N-trimethylammonium bromide (CTAB), at pH 8.0 and the activity was found to have almost doubled. The concentration of CTAB, the time and temperature of treatment were optimised for maximum enzyme activity and were found to be 0.2%, 20 min and 5-degrees-C respectively. Subsequently, the cell bound activity was retained for a longer period by chemical cross-linking with 0.1% glutaraldehyde.

引用

页码：431 / 434

页数：4

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