EVIDENCE FOR EPOXIDASE ACTIVITY IN DEACETOXY DEACETYLCEPHALOSPORIN-C SYNTHASE

被引：7

作者：

BALDWIN, JE ^{[1
]}

ADLINGTON, RM ^{[1
]}

CROUCH, NP ^{[1
]}

PEREIRA, IAC ^{[1
]}

机构：

[1] UNIV OXFORD,OXFORD CTR MOLEC SCI,OXFORD OX1 3QY,ENGLAND

来源：

TETRAHEDRON | 1993年 / 49卷 / 22期

关键词：

D O I：

10.1016/S0040-4020(01)80408-7

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

The conversion of the unnatural substrate exomethylene cephalosporin C by deacetoxy/deacetylcephalosporin C synthase (DAOC/DACS) has been studied by the use of competitive kinetic isotope experiments. These suggest that the first irreversible event in this conversion does not involve C4-hydrogen abstraction. A novel epoxide cepham metabolite has been isolated from the enzymic conversion of [4-H-2]exomethylene cephalosporin C. It is believed that increased formation of this shunt metabolite is caused by the operation of a deuterium kinetic isotope effect on an enzyme-bound intermediate. The epoxide cepham is also accepted as a substrate by DAOC/DACS, being oxidised to an aldehyde product.

引用

页码：4907 / 4922

页数：16

共 25 条

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