SYNTHESIS, CHARACTERIZATION AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF C6-C16 DICARBOXYLYL-MONO-COENZYME-A AND DICARBOXYLYL-MONO-CARNITINE ESTERS

被引：15

作者：

POURFARZAM, M

BARTLETT, K

机构：

[1] UNIV NEWCASTLE UPON TYNE,SCH MED,DEPT CHILD HLTH,FRAMLINGTON PL,NEWCASTLE TYNE NE2 4HH,ENGLAND

[2] UNIV NEWCASTLE UPON TYNE,SCH MED,DEPT CLIN BIOCHEM,NEWCASTLE TYNE NE2 4HH,ENGLAND

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 570卷 / 02期

关键词：

D O I：

10.1016/0378-4347(91)80529-L

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The synthesis and purification of the mono-coenzyme A and mono-carnitine esters of the homologous series of straight-chain even-numbered dicarboxylic acids (C6-C-16) is described. The corresponding 3-hydroxyacyl- and 2-enoyl-CoA esters were prepared enzymatically. A reversed-phase high-performance liquid chromatographic (HPLC) system for the analysis of the intact CoA esters is described and their chromatographic behaviour documented. Reversed-phase HPLC systems for the analysis of the 4-bromophenacyl derivatives of the dicarboxylyl-mono-carnitines and the 4-nitrobenzyl derivatives of the free acids are also described. Some preliminary studies of the metabolism of [U-C-14]hexadecanedionoyl-mono-CoA by rat liver peroxisomes and rat skeletal muscle mitochondria are described illustrating the application of these methods.

引用

页码：253 / 276

页数：24

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