OBSERVATION OF INTACT (HEME-BOUND) MYOGLOBIN BY ELECTROSPRAY IONIZATION ON A DOUBLE-FOCUSING MASS-SPECTROMETER

被引：39

作者：

LOO, JA ^{[1
]}

GIORDANI, AB ^{[1
]}

MUENSTER, H ^{[1
]}

机构：

[1] FINNIGAN MAT GMBH,BREMEN,GERMANY

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1993年 / 7卷 / 03期

关键词：

D O I：

10.1002/rcm.1290070304

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A noncovalently bound complex, the heme-apomyoglobin protein system (M(r) 17 568), was detected using electrospray ionization on a double-focusing mass spectrometer with an array detector. The kilovolt energy conditions used for ion transmission and focusing did not cause significant amounts of fragmentation of the weakly-bound complex. With a high-performance array detector, femtomolar sensitivity was achieved for myoglobin in water.

引用

页码：186 / 189

页数：4

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