LIPID CLASS ANALYSIS BY NORMAL-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, DEVELOPMENT AND OPTIMIZATION USING MULTIVARIATE METHODS

被引：37

作者：

ARNOLDSSON, KC

KAUFMANN, P

机构：

[1] KARLSHAMNS LIPIDTEKN AB,POB 6686,S-11384 STOCKHOLM,SWEDEN

[2] UNIV STOCKHOLM,DEPT ANALYT CHEM,S-10691 STOCKHOLM,SWEDEN

来源：

CHROMATOGRAPHIA | 1994年 / 38卷 / 5-6期

关键词：

COLUMN LIQUID CHROMATOGRAPHY; LIPID CLASS ANALYSIS; EXPERIMENTAL DESIGN AND OPTIMIZATION; CHEMOMETRICS;

D O I：

10.1007/BF02269774

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A general method for the analysis of lipid classes by liquid chromatography has been developed using a multivariate optimization strategy to target optimal system conditions. The method was validated using a ruggedness test in the form of a Plackett-Burman design thereby exploring the immediate region around the optimum to ensure stable analytical conditions. Detection and quantitation were optimized by a factorial design in the light scattering detector parameters thus ensuring maximum detector response. This method was found to be suitable for a broad range of lipid sources from vegetable and animal origin, examples of the separation achieved are given for oat kernel, soybean and bovine milk lipids.

引用

页码：317 / 324

页数：8

共 31 条

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