CHIRAL SEPARATIONS OF BETA-BLOCKING DRUG SUBSTANCES USING CHIRAL STATIONARY PHASES

Three chiral stationary phases were evaluated for their usefulness in determinations of optical purity and separations of P-blocking drug substances. The phases were a modified cellulose phase (Chiralcel OD), an immobilized protein (AGP) and a P-cyclodextrin phase (Cyclobond I). These phases were evaluated concerning their practical analytical use for the determination of the enantiomeric purity of non-racemic P-blocking substances. The best phase proved to be Chiralcel OD, which provided excellent separations of almost all P-blocking substances tested and rugged determinations performed with satisfactorily low detection Limits. Similar results could be obtained with AGP, but this did not seem to be sufficiently rugged for routine analysis. The P-cyclodextrin phase resulted in poor chiral separations of most P-blocking substances and the usefulness of this phase for analytical purposes seems limited.