ACTION PATTERN OF ALPHA-AMYLASE FROM ASPERGILLUS-ORYZAE IN CONCENTRATED MEDIA

被引：13

作者：

GRABER, M

COMBES, D

机构：

[1] Département de Génie Biochimique Et Alimentaire, Ua-Cnrs 544, Institut National Des Sciences Appliquées, Toulouse, F-31077, Avenue de Rangueil

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1990年 / 36卷 / 01期

关键词：

D O I：

10.1002/bit.260360103

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The influence of concentrated maltotetraose solutions on fungal alpha‐amylase activity and specificity has been determined. It has been found that the enzyme is not inhibited by 500 g/L substrate concentration and that transglycosylation reactions are increased with rising substrate concentration. The amount of oligosaccharides of polymerization degree higher than maltotetraose reaches 20% (w/w). Moreover, the effect of polyhydric alcohols, known as water activity depressors has been analyzed: the presence of polyols does not modify the amount of transglycosylation products, but changes the hydrolysis pattern by favoring the formation of low polymerization degree oligosaccharides. Copyright © 1990 John Wiley & Sons, Inc.

引用

页码：12 / 18

页数：7

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