SUPPRESSION OF HERPES-SIMPLEX VIRUS TYPE-1 REACTIVATION FROM LATENCY BY (+/-)-9-([(Z)-2-(HYDROXYMETHYL)CYCLOHEXYL]METHYL) GUANINE (L-653,180) INVITRO

Latent herpes simplex virus type 1 (HSV-1) infection was induced in human embryonic lung cells in vitro by using a combination of viral replication inhibitors and elevated temperature. Under reactivating conditions (superinfection by human cytomegalovirus or temperature manipulation), a nonantiviral thymidine kinase inhibitor (L-653,180) was found to suppress or delay reactivation of HSV-1 from latently infected human embryonic lung cells. L-653,180 alone or in combination with interferon was ineffective as a primary or acute viral replication inhibitor and was unable to induce latent HSV-1 infection in cell culture. These data suggest that initial or acute virus replication and replication resulting from reactivation from latency are separate events.