SIMULTANEOUS HYDROLYSIS OF TRISACCHARIDES AND TETRASACCHARIDES BY INDUSTRIAL MIXTURES OF GLUCOAMYLASE AND ALPHA-AMYLASE - KINETICS AND THERMODYNAMICS

被引：3

作者：

CONVERTI, A

FIORITO, G

DELBORGHI, M

FERRAIOLO, G

机构：

[1] Institute of Chemical Engineering Science and Technology, University of Genoa, Genoa, I-16145, Via Opera Pia

来源：

BIOPROCESS ENGINEERING | 1991年 / 7卷 / 04期

关键词：

D O I：

10.1007/BF00387412

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The present paper deals with the study of the kinetics and thermodynamics of batch enzymic hydrolysis of multisubstrate media in the presence of more than one glucanase. A kinetic model, similar to the one proposed for competitive inhibition, is presented to describe the competition between two substrates, tri- and tetrasaccharides, for the same enzyme, glucoamylase. A literature research on the most recurrent values of the Michaelis-Menten constant also shows a linear relationship between this parameter and the molecular weight of the sugar substrate for a given glucanase.

引用

页码：165 / 170

页数：6

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